Hotspot

accD-psaI

LSC · 104849-105242 · 394 bp

Back to Barfussia

Pi 0.0102

MarkerSeek score 49.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 4
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	506-508 bp	1.0	79.0
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	505-507 bp	1.0	79.0
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	512-514 bp	1.0	78.3
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	511-513 bp	1.0	78.3
accD-psaI_p5	GCTCCATGGTTTCTTTCCCT	CCGGAAATACTAGGCCCACT	50.0	55.0	57.8	58.9	493-495 bp	1.0	72.2