Hotspot

atpB-rbcL

LSC · 100475-101254 · 780 bp

Back to Barfussia

Pi 0.00129

MarkerSeek score 60.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	914-918 bp	1.0	78.8
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	892-896 bp	1.0	78.5
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.1	58.4	915-919 bp	1.0	76.4
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	893-897 bp	1.0	76.1
atpB-rbcL_p5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	893-897 bp	1.0	75.6