Hotspot

trnC-GCA-petN

LSC · 73427-74454 · 1028 bp

Back to Barfussia

Pi 0.00781

MarkerSeek score 61.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 8
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.5	58.1	1132-1136 bp	1.0	76.1
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	50.0	55.0	58.5	58.1	1131-1135 bp	1.0	76.1
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1073-1077 bp	1.0	75.9
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	GAGTCCACTCCTTCCCCATA	50.0	55.0	58.5	57.8	1133-1137 bp	1.0	75.6
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	CTAGAGTCCACTCCTTCCCC	50.0	60.0	58.5	58.2	1136-1140 bp	1.0	75.3