Hotspot

atpH-atpI

LSC · 58626-59711 · 1086 bp

Back to Barfussia

Pi 0.00296

MarkerSeek score 64.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 3
pi: 0.00296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1070-1143 bp	1.0	82.5
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	1129-1202 bp	1.0	82.2
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1086-1159 bp	1.0	81.6
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1071-1144 bp	1.0	81.3
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1100-1173 bp	1.0	81.3