Hotspot

atpF-atpH

LSC · 57941-58379 · 439 bp

Back to Barfussia

Pi 0.00911

MarkerSeek score 60.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 4
pi: 0.00911
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCGTGAGAAAACGAAAGA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	58.1	57.9	519 bp	1.0	79.6
atpF-atpH_p2	TATTAAACCCGAAACCCCCG	GTTGTGGCATTAGCGCTTTT	50.0	45.0	57.6	57.9	551 bp	1.0	76.7
atpF-atpH_p3	TGGCGTGAGAAAACGAAAGA	ATTTACGGACTGGTTGTGGC	45.0	50.0	58.1	58.5	531 bp	1.0	76.4
atpF-atpH_p4	CAATGGCGTGAGAAAACGAA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	57.3	57.9	522 bp	1.0	74.7
atpF-atpH_p5	TATTAAACCCGAAACCCCCG	ATTTACGGACTGGTTGTGGC	50.0	50.0	57.6	58.5	563 bp	1.0	73.5