Hotspot

ycf15-ycf2

IRb · 35530-35597 · 68 bp

Back to Barfussia

Pi 0.0147

MarkerSeek score 61.00

Primer available yes

Label ycf15-ycf2

Feature Metadata

label_name: ycf15-ycf2
feature_type: igs
region: IRb
length_bp: 68
variable_sites: 1
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-ycf2_p1	TCCATGTTTCAGCAGTAGCA	AAAGAGATGGCTTTTCCCGG	45.0	50.0	57.1	58.2	214 bp	1.0	72.2
ycf15-ycf2_p2	TCCATGTTTCAGCAGTAGCA	ATGGCTTTTCCCGGATGAAA	45.0	45.0	57.1	57.8	208 bp	1.0	71.7
ycf15-ycf2_p3	TCCATGTTTCAGCAGTAGCA	TGGCTTTTCCCGGATGAAAT	45.0	45.0	57.1	57.8	207 bp	1.0	71.7
ycf15-ycf2_p4	TCCATGTTTCAGCAGTAGCA	GATGGCTTTTCCCGGATGAA	45.0	50.0	57.1	58.2	209 bp	1.0	71.6
ycf15-ycf2_p5	TGTTCCATGGAGCTAAGGTC	AAAGAGATGGCTTTTCCCGG	50.0	50.0	56.9	58.2	191 bp	1.0	70.5