primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.532399	3356	4133	851-865	1.000000	77.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.533769	3356	4133	852-866	1.000000	77.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.548744	3356	4133	911-925	1.000000	77.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.6	58.0	6.3	0.0	0.593672	3356	4133	854-868	1.000000	76.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.6	58.0	6.3	0.0	0.593672	3356	4133	855-869	1.000000	76.8
ycf15-ycf2_p1	ycf15-ycf2	1	TCCATGTTTCAGCAGTAGCA	AAAGAGATGGCTTTTCCCGG	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.017762	35530	35597	214-214	1.000000	72.2
ycf15-ycf2_p2	ycf15-ycf2	2	TCCATGTTTCAGCAGTAGCA	ATGGCTTTTCCCGGATGAAA	20	20	45.0	45.0	57.1	57.8	0.0	0.7	1.087315	35530	35597	208-208	1.000000	71.7
ycf15-ycf2_p3	ycf15-ycf2	3	TCCATGTTTCAGCAGTAGCA	TGGCTTTTCCCGGATGAAAT	20	20	45.0	45.0	57.1	57.8	0.0	0.7	1.087315	35530	35597	207-207	1.000000	71.7
ycf15-ycf2_p4	ycf15-ycf2	4	TCCATGTTTCAGCAGTAGCA	GATGGCTTTTCCCGGATGAA	20	20	45.0	50.0	57.1	58.2	0.0	0.7	1.094478	35530	35597	209-209	1.000000	71.6
ycf15-ycf2_p5	ycf15-ycf2	5	TGTTCCATGGAGCTAAGGTC	AAAGAGATGGCTTTTCCCGG	20	20	50.0	50.0	56.9	58.2	26.6	0.0	1.241002	35530	35597	191-191	1.000000	70.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GAGAAAGCGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.0	55.5	0.0	0.0	3.552980	52656	53864	1336-1338	1.000000	53.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	52656	53864	1281-1283	1.000000	53.3
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GAGAAAGCGGAAAGAGAGGG	AGAACGAATCACACTTTTACCAC	20	23	55.0	39.1	58.0	57.3	0.0	0.0	3.685696	52656	53864	1339-1341	1.000000	52.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GAGAAAGCGGAAAGAGAGGG	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.0	57.3	0.0	0.0	3.685696	52656	53864	1338-1340	1.000000	52.3
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	52656	53864	1283-1285	1.000000	52.2
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	GAAAGGGGCGAGTGAAGTAA	20	20	45.0	50.0	57.4	57.5	9.6	0.0	1.109704	56552	57940	1567-1568	1.000000	71.4
atpF_p2	atpF	2	GGCTCGAAGGGTTACCATTA	GAAAGGGGCGAGTGAAGTAA	20	20	50.0	50.0	57.0	57.5	0.0	0.0	1.461751	56552	57940	1555-1556	1.000000	68.6
atpF_p3	atpF	3	TCTTTTTCGGAAGCAAAGGGA	GAAAGGGGCGAGTGAAGTAA	21	20	42.9	50.0	58.1	57.5	12.6	0.0	1.528728	56552	57940	1523-1524	1.000000	68.1
atpF_p4	atpF	4	GGCTCGAAGGGTTACCATTAG	GAAAGGGGCGAGTGAAGTAA	21	20	52.4	50.0	58.2	57.5	0.0	0.0	1.632539	56552	57940	1555-1556	1.000000	67.2
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	GAAAGGGGCGAGTGAAGTAAT	20	21	45.0	47.6	57.4	58.0	9.6	0.0	1.654906	56552	57940	1567-1568	1.000000	67.1
atpF-atpH_p1	atpF-atpH	1	TGGCGTGAGAAAACGAAAGA	GTTGTGGCATTAGCGCTTTT	20	20	45.0	45.0	58.1	57.9	0.2	18.7	0.121896	57941	58379	519-519	1.000000	79.6
atpF-atpH_p2	atpF-atpH	2	TATTAAACCCGAAACCCCCG	GTTGTGGCATTAGCGCTTTT	20	20	50.0	45.0	57.6	57.9	0.0	18.7	0.481253	57941	58379	551-551	1.000000	76.7
atpF-atpH_p3	atpF-atpH	3	TGGCGTGAGAAAACGAAAGA	ATTTACGGACTGGTTGTGGC	20	20	45.0	50.0	58.1	58.5	0.2	0.0	0.526999	57941	58379	531-531	1.000000	76.4
atpF-atpH_p4	atpF-atpH	4	CAATGGCGTGAGAAAACGAA	GTTGTGGCATTAGCGCTTTT	20	20	45.0	45.0	57.3	57.9	0.2	18.7	0.743928	57941	58379	522-522	1.000000	74.7
atpF-atpH_p5	atpF-atpH	5	TATTAAACCCGAAACCCCCG	ATTTACGGACTGGTTGTGGC	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.886356	57941	58379	563-563	1.000000	73.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	58626	59711	1070-1143	1.000000	82.5
atpH-atpI_p2	atpH-atpI	2	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	20	20	55.0	55.0	57.9	57.8	0.0	21.3	0.256164	58626	59711	1129-1202	1.000000	82.2
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	57.9	57.8	0.0	21.3	0.324323	58626	59711	1086-1159	1.000000	81.6
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.2	57.8	1.4	21.3	0.359803	58626	59711	1071-1144	1.000000	81.3
atpH-atpI_p5	atpH-atpI	5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.360882	58626	59711	1100-1173	1.000000	81.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	58.4	58.1	0.0	14.6	0.480541	72006	73355	1166-1417	1.000000	80.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	57.6	58.1	0.0	14.6	0.506504	72006	73355	1167-1418	1.000000	80.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.885652	72006	73355	1192-1443	1.000000	77.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.911615	72006	73355	1193-1444	1.000000	76.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	20	20	45.0	50.0	57.1	58.1	10.9	14.6	0.930297	72006	73355	1243-1494	1.000000	76.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	20	20	50.0	55.0	58.5	58.1	0.0	0.0	0.595302	73427	74454	1132-1136	1.000000	76.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	20	20	50.0	55.0	58.5	58.1	0.0	0.0	0.595302	73427	74454	1131-1135	1.000000	76.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	73427	74454	1073-1077	1.000000	75.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	AGTTCAAATCTGGGTGTCGC	GAGTCCACTCCTTCCCCATA	20	20	50.0	55.0	58.5	57.8	0.0	0.0	0.652490	73427	74454	1133-1137	1.000000	75.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	AGTTCAAATCTGGGTGTCGC	CTAGAGTCCACTCCTTCCCC	20	20	50.0	60.0	58.5	58.2	0.0	3.0	0.693171	73427	74454	1136-1140	1.000000	75.3
petN-psbM_p1	petN-psbM	1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	74545	75793	1353-1353	1.000000	68.3
petN-psbM_p2	petN-psbM	2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	74545	75793	1352-1352	1.000000	68.3
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	74545	75793	1411-1411	1.000000	68.0
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.8	57.6	0.0	0.0	1.583671	74545	75793	1351-1351	1.000000	67.8
petN-psbM_p5	petN-psbM	5	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	20	21	60.0	42.9	58.2	57.6	6.7	0.0	1.624352	74545	75793	1348-1348	1.000000	67.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	94284	95054	876-881	1.000000	79.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	94284	95054	866-871	1.000000	78.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	94284	95054	919-924	1.000000	77.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	94284	95054	918-923	1.000000	77.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.2	58.2	0.0	7.9	0.407965	94284	95054	908-913	1.000000	77.3
atpB-rbcL_p1	atpB-rbcL	1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.213422	100475	101254	914-918	1.000000	78.8
atpB-rbcL_p2	atpB-rbcL	2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	57.9	58.1	2.1	0.0	0.255358	100475	101254	892-896	1.000000	78.5
atpB-rbcL_p3	atpB-rbcL	3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.514303	100475	101254	915-919	1.000000	76.4
atpB-rbcL_p4	atpB-rbcL	4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	57.9	58.4	2.1	0.0	0.556239	100475	101254	893-897	1.000000	76.1
atpB-rbcL_p5	atpB-rbcL	5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.5	58.1	8.0	0.0	0.616166	100475	101254	893-897	1.000000	75.6
accD-psaI_p1	accD-psaI	1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	104849	105242	506-508	1.000000	79.0
accD-psaI_p2	accD-psaI	2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	104849	105242	505-507	1.000000	79.0
accD-psaI_p3	accD-psaI	3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	104849	105242	512-514	1.000000	78.3
accD-psaI_p4	accD-psaI	4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	104849	105242	511-513	1.000000	78.3
accD-psaI_p5	accD-psaI	5	GCTCCATGGTTTCTTTCCCT	CCGGAAATACTAGGCCCACT	20	20	50.0	55.0	57.8	58.9	12.6	0.0	1.093169	104849	105242	493-495	1.000000	72.2
ycf4-cemA_p1	ycf4-cemA	1	ACAAAAAGCTGCCGAATTGG	TGGGAAAGAAGGCAATGCTT	20	20	45.0	45.0	57.8	58.0	0.0	4.8	0.167376	106247	107097	939-939	1.000000	78.8
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCCGAATTGG	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.311006	106247	107097	985-985	1.000000	77.7
ycf4-cemA_p3	ycf4-cemA	3	GCCATTCCCTTGACTCGTAC	TGGGAAAGAAGGCAATGCTT	20	20	55.0	45.0	58.3	58.0	0.0	4.8	0.360858	106247	107097	989-989	1.000000	77.3
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCCGAATTGG	ATGGGAAAGAAGGCAATGCT	20	20	45.0	45.0	57.8	57.8	0.0	4.8	0.396249	106247	107097	940-940	1.000000	77.0
ycf4-cemA_p5	ycf4-cemA	5	GCCATTCCCTTGACTCGTAC	ATGAGAGAGAGACCCACCAG	20	20	55.0	55.0	58.3	57.8	0.0	0.0	0.504488	106247	107097	1035-1035	1.000000	76.1
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	108981	110096	1242-1265	1.000000	79.5
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	108981	110096	1240-1263	1.000000	79.5
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.461827	108981	110096	1241-1264	1.000000	76.6
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	108981	110096	1249-1272	1.000000	74.4
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	108981	110096	1247-1270	1.000000	74.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	110866	112394	1288-1676	1.000000	78.5
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	110866	112394	1287-1675	1.000000	78.5
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	110866	112394	1301-1689	1.000000	78.2
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	110866	112394	1300-1688	1.000000	78.2
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	20	20	45.0	45.0	58.2	57.3	0.0	0.0	0.827968	110866	112394	1296-1684	1.000000	77.5
rpl16_p1	rpl16	1	TCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGCT	20	26	45.0	30.8	56.6	57.6	0.0	0.0	7.802716	127916	129414	1557-1559	1.000000	40.4
rpl16_p2	rpl16	2	TCCCCAATACCCCTTTTTGTT	TCGATTGATCATTTGGTATAATTGCT	21	26	42.9	30.8	57.4	57.6	0.0	0.0	8.034596	127916	129414	1557-1559	1.000000	40.4
rpl16_p3	rpl16	3	TCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGC	20	25	45.0	32.0	56.6	56.4	0.0	0.0	8.037656	127916	129414	1557-1559	1.000000	40.4
rpl16_p4	rpl16	4	TCCCCAATACCCCTTTTTGT	CGATTGATCATTTGGTATAATTGCT	20	25	45.0	32.0	56.6	56.4	0.0	0.0	8.045563	127916	129414	1556-1558	1.000000	40.4
rpl16_p5	rpl16	5	ATCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGCT	21	26	42.9	30.8	57.1	57.6	0.0	0.0	8.259527	127916	129414	1558-1560	1.000000	40.4
ycf2-ycf15_p1	ycf2-ycf15	1	AAAGAGATGGCTTTTCCCGG	TCCATGTTTCAGCAGTAGCA	20	20	50.0	45.0	58.2	57.1	0.0	0.0	1.017762	140248	140315	214-214	1.000000	72.2
ycf2-ycf15_p2	ycf2-ycf15	2	ATGGCTTTTCCCGGATGAAA	TCCATGTTTCAGCAGTAGCA	20	20	45.0	45.0	57.8	57.1	0.7	0.0	1.087315	140248	140315	208-208	1.000000	71.7
ycf2-ycf15_p3	ycf2-ycf15	3	TGGCTTTTCCCGGATGAAAT	TCCATGTTTCAGCAGTAGCA	20	20	45.0	45.0	57.8	57.1	0.7	0.0	1.087315	140248	140315	207-207	1.000000	71.7
ycf2-ycf15_p4	ycf2-ycf15	4	GATGGCTTTTCCCGGATGAA	TCCATGTTTCAGCAGTAGCA	20	20	50.0	45.0	58.2	57.1	0.7	0.0	1.094478	140248	140315	209-209	1.000000	71.6
ycf2-ycf15_p5	ycf2-ycf15	5	AAAGAGATGGCTTTTCCCGG	TGTTCCATGGAGCTAAGGTC	20	20	50.0	50.0	58.2	56.9	0.0	26.6	1.241002	140248	140315	191-191	1.000000	70.5