Hotspot

ycf1

Genome · 129179-134857 · 5679 bp

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Pi 0.00441

MarkerSeek score 55.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5679
variable_sites: 25
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCTCGTGTATGGAAGTAGTCA	ACGAAATAAAGGAAAAGGGTGC	45.5	40.9	58.0	57.5	311 bp	1.0	44.6
ycf1_p2	TCCTCGTGTATGGAAGTAGTCA	GGGTGCTAATAAAACGAATCCC	45.5	45.5	58.0	57.4	295 bp	1.0	44.3
ycf1_p3	TCCTCGTGTATGGAAGTAGTCA	CGAAATAAAGGAAAAGGGTGCT	45.5	40.9	58.0	57.2	310 bp	1.0	42.4
ycf1_p4	AATTCCAGAATTCCGCAGTT	ACGAAATAAAGGAAAAGGGTGC	40.0	40.9	55.6	57.5	359 bp	1.0	41.7
ycf1_p5	ATTCCAGAATTCCGCAGTTT	ACGAAATAAAGGAAAAGGGTGC	40.0	40.9	55.6	57.5	358 bp	1.0	41.7