Hotspot

ndhF-rpl32

Genome · 117665-118769 · 1105 bp

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Pi 0.00182

MarkerSeek score 60.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1105
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAATAAGCA	TTTACCGCTGCCCAATATCC	45.0	50.0	57.8	58.0	1293-1299 bp	1.0	78.9
ndhF-rpl32_p2	GTCGGAATAAGCAGGAGTCC	TTTACCGCTGCCCAATATCC	55.0	50.0	57.8	58.0	1286-1292 bp	1.0	78.5
ndhF-rpl32_p3	TTGCTGTCGGAATAAGCAGG	TTTACCGCTGCCCAATATCC	50.0	50.0	58.3	58.0	1291-1297 bp	1.0	78.2
ndhF-rpl32_p4	AAGCAGGAGTCCCAATCCTA	TTTACCGCTGCCCAATATCC	50.0	50.0	57.7	58.0	1278-1284 bp	1.0	78.1
ndhF-rpl32_p5	TGTTGCTGTCGGAATAAGCA	AAAAAGCTTTTACCGCTGCC	45.0	45.0	57.8	57.9	1301-1307 bp	1.0	77.9