Hotspot

ycf1-ndhF

Genome · 115389-115432 · 44 bp

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Pi 0.0263

MarkerSeek score 69.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: Genome
length_bp: 44
variable_sites: 1
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AATTCGGTCGTTATGGTCGG	TATAGGGGGAGGGCGAATTT	50.0	50.0	58.1	57.9	219-234 bp	1.0	82.8
ycf1-ndhF_p2	AATTCGGTCGTTATGGTCGG	GGGAGGGCGAATTTCTTCTT	50.0	50.0	58.1	57.9	213-228 bp	1.0	82.8
ycf1-ndhF_p3	AATTCGGTCGTTATGGTCGG	ATATAGGGGGAGGGCGAATT	50.0	50.0	58.1	57.6	220-235 bp	1.0	80.9
ycf1-ndhF_p4	GGTCGTTATGGTCGGACTTT	TATAGGGGGAGGGCGAATTT	50.0	50.0	57.6	57.9	214-229 bp	1.0	80.3
ycf1-ndhF_p5	GGTCGTTATGGTCGGACTTT	GGGAGGGCGAATTTCTTCTT	50.0	50.0	57.6	57.9	208-223 bp	1.0	80.3