Hotspot

DR443_pgt019-DR443_pgt020

Genome · 72280-72422 · 143 bp

Back to Barclaya

Pi 0.014
MarkerSeek score 60.50
Primer available yes
Label DR443_pgt019-DR443_pgt020

Feature Metadata

label_name
DR443_pgt019-DR443_pgt020
feature_type
igs
region
Genome
length_bp
143
variable_sites
2
pi
0.014
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR443_pgt019-DR443_pgt020_p1 GCGTTCTACCGAACTGAACT GGATGTAGCGCAGTTTGGTA 50.0 50.0 57.9 58.0 243 bp 1.0 80.0
DR443_pgt019-DR443_pgt020_p2 ACCTACGACATCGGGTTTTG GGATGTAGCGCAGTTTGGTA 50.0 50.0 57.9 58.0 270 bp 1.0 79.7
DR443_pgt019-DR443_pgt020_p3 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 210 bp 1.0 79.5
DR443_pgt019-DR443_pgt020_p4 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 211 bp 1.0 79.5
DR443_pgt019-DR443_pgt020_p5 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 199 bp 1.0 79.4