Hotspot

psbC-DR443_pgt011

Genome · 38896-39105 · 210 bp

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Pi 0.0286
MarkerSeek score 68.90
Primer available yes
Label psbC-DR443_pgt011

Feature Metadata

label_name
psbC-DR443_pgt011
feature_type
igs
region
Genome
length_bp
210
variable_sites
6
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-DR443_pgt011_p1 CTGCGGCAGGTTTTGAAAAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.1 58.0 373 bp 1.0 80.6
psbC-DR443_pgt011_p2 TGCGGCAGGTTTTGAAAAAG GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.1 58.0 372 bp 1.0 80.6
psbC-DR443_pgt011_p3 CTGCGGCAGGTTTTGAAAAA GCTGAGTGGTTGATAGCTCC 45.0 55.0 58.1 58.1 365 bp 1.0 80.4
psbC-DR443_pgt011_p4 TGCGGCAGGTTTTGAAAAAG GCTGAGTGGTTGATAGCTCC 45.0 55.0 58.1 58.1 364 bp 1.0 80.4
psbC-DR443_pgt011_p5 TGCGGCAGGTTTTGAAAAAG GGCTGAGTGGTTGATAGCTC 45.0 55.0 58.1 58.1 365 bp 1.0 80.4