Hotspot

DR443_pgt008-DR443_pgt009

Genome · 33532-33947 · 416 bp

Back to Barclaya

Pi 0.012
MarkerSeek score 57.60
Primer available yes
Label DR443_pgt008-DR443_pgt009

Feature Metadata

label_name
DR443_pgt008-DR443_pgt009
feature_type
igs
region
Genome
length_bp
416
variable_sites
5
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR443_pgt008-DR443_pgt009_p1 TTCATAGGATCCCTGGTCGG GGGGACGGACTGTAAATTCG 55.0 55.0 58.3 58.4 573-578 bp 1.0 75.8
DR443_pgt008-DR443_pgt009_p2 CATAGGATCCCTGGTCGGTA GGGGACGGACTGTAAATTCG 55.0 55.0 57.7 58.4 571-576 bp 1.0 75.6
DR443_pgt008-DR443_pgt009_p3 ATAGGATCCCTGGTCGGTAC GGGGACGGACTGTAAATTCG 55.0 55.0 57.7 58.4 570-575 bp 1.0 75.6
DR443_pgt008-DR443_pgt009_p4 TTCATAGGATCCCTGGTCGG GGGACGGACTGTAAATTCGT 55.0 50.0 58.3 57.6 572-577 bp 1.0 75.6
DR443_pgt008-DR443_pgt009_p5 CATAGGATCCCTGGTCGGTA GGGACGGACTGTAAATTCGT 55.0 50.0 57.7 57.6 570-575 bp 1.0 75.4