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psbM-DR443_pgt008

Genome · 32664-33457 · 794 bp

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Pi 0.00385

MarkerSeek score 58.40

Primer available yes

Label psbM-DR443_pgt008

Feature Metadata

label_name: psbM-DR443_pgt008
feature_type: igs
region: Genome
length_bp: 794
variable_sites: 3
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-DR443_pgt008_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	888-902 bp	1.0	75.6
psbM-DR443_pgt008_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	887-901 bp	1.0	74.7
psbM-DR443_pgt008_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	889-903 bp	1.0	73.9
psbM-DR443_pgt008_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	891-905 bp	1.0	72.6
psbM-DR443_pgt008_p5	ACTAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	894-908 bp	1.0	71.7