Hotspot

DR443_pgt001-psbA

Genome · 72-559 · 488 bp

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Pi 0.0541
MarkerSeek score 85.00
Primer available yes
Label DR443_pgt001-psbA

Feature Metadata

label_name
DR443_pgt001-psbA
feature_type
igs
region
Genome
length_bp
488
variable_sites
24
pi
0.0541
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR443_pgt001-psbA_p1 AATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.7 57.9 518-554 bp 1.0 84.4
DR443_pgt001-psbA_p2 AATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.7 57.9 521-557 bp 1.0 84.3
DR443_pgt001-psbA_p3 AATCCACTTGGCTACATCCG TAGACCTAGCTGCTGTCGAA 50.0 50.0 57.7 57.9 514-550 bp 1.0 84.0
DR443_pgt001-psbA_p4 AATCCACTTGGCTACATCCG CTAGACCTAGCTGCTGTCGA 50.0 55.0 57.7 58.3 515-551 bp 1.0 82.3
DR443_pgt001-psbA_p5 AATCCACTTGGCTACATCCG TCTAGACCTAGCTGCTGTCG 50.0 55.0 57.7 58.3 516-552 bp 1.0 82.3