Hotspot

ycf1

SSC · 124035-129416 · 5382 bp

Back to Barbarea

Pi 0.00707

MarkerSeek score 50.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 57
pi: 0.00707
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	AATGAAGCCGTGGGTCATAC	45.0	50.0	58.0	58.0	362 bp	1.0	81.3
ycf1_p2	TCCATCGCAATTGAAAACGC	AAGTGTTTTTGAGGCCGACT	45.0	45.0	58.0	57.9	833 bp	1.0	80.6
ycf1_p3	ACAGACAAACGGACAGGTTC	AATGAAGCCGTGGGTCATAC	50.0	50.0	58.1	58.0	564 bp	1.0	80.6
ycf1_p4	CAGACAAACGGACAGGTTCA	AATGAAGCCGTGGGTCATAC	50.0	50.0	58.1	58.0	563 bp	1.0	80.6
ycf1_p5	TCCATCGCAATTGAAAACGC	ACGTCTATAGGCCTTGCTCT	45.0	50.0	58.0	57.9	721 bp	1.0	80.5