Hotspot

rpl32-trnL-UAG

SSC · 113536-114442 · 907 bp

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Pi 0.014

MarkerSeek score 56.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 907
variable_sites: 19
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTGGACGTCGTTGAAAGCT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	1033-1041 bp	1.0	79.8
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACGTCG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	57.8	57.9	1042-1050 bp	1.0	79.6
rpl32-trnL-UAG_p3	ATTGGACGTCGTTGAAAGCT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	1026-1034 bp	1.0	79.3
rpl32-trnL-UAG_p4	ATTGGACGTCGTTGAAAGCT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.1	57.8	1032-1040 bp	1.0	79.2
rpl32-trnL-UAG_p5	ATTGGACGTCGTTGAAAGCT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	1031-1039 bp	1.0	79.2