Hotspot

petD-rpoA

LSC · 77293-77493 · 201 bp

Back to Barbarea

Pi 0.0166

MarkerSeek score 64.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 5
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCA	50.0	36.4	57.9	55.6	383-396 bp	1.0	46.9
petD-rpoA_p2	CTTTTTGATTGGCACCGTGG	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.2	55.6	379-392 bp	1.0	45.7
petD-rpoA_p3	CAGTAGCGACAACCGTCTTT	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.2	55.6	395-408 bp	1.0	45.6
petD-rpoA_p4	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCAAG	50.0	37.5	57.9	57.4	383-396 bp	1.0	45.3
petD-rpoA_p5	CGTCTTTTTGATTGGCACCG	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.3	55.6	382-395 bp	1.0	44.9