Hotspot

trnP-UGG-psaJ

LSC · 66098-66470 · 373 bp

Back to Barbarea

Pi 0.00894

MarkerSeek score 52.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 5
pi: 0.00894
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCCCTCGTTACTGCTCAGAA	AACGAAGCGAACCATAGAGT	50.0	45.0	58.1	57.0	545 bp	1.0	72.0
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	AACGAAGCGAACCATAGAGT	50.0	45.0	58.1	57.0	493 bp	1.0	71.9
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	AACGAAGCGAACCATAGAGT	50.0	45.0	58.1	57.0	492 bp	1.0	71.9
trnP-UGG-psaJ_p4	ACAGGATTTGAACCCGTGAC	AACGAAGCGAACCATAGAGT	50.0	45.0	58.1	57.0	504 bp	1.0	71.8
trnP-UGG-psaJ_p5	CAGGATTTGAACCCGTGACA	AACGAAGCGAACCATAGAGT	50.0	45.0	58.1	57.0	503 bp	1.0	71.8