Hotspot

petA-psbJ

LSC · 62488-63497 · 1010 bp

Back to Barbarea

Pi 0.0126

MarkerSeek score 53.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 19
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1709-1717 bp	1.0	81.0
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1708-1716 bp	1.0	81.0
petA-psbJ_p3	GTTTCAGAGGGCGAATCCAT	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1806-1814 bp	1.0	80.9
petA-psbJ_p4	GTTTCAGAGGGCGAATCCAT	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1805-1813 bp	1.0	80.9
petA-psbJ_p5	CGGACGCCAAGTGATTGATA	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	1851-1859 bp	1.0	80.7