Hotspot

accD-psaI

LSC · 58452-59108 · 657 bp

Back to Barbarea

Pi 0.00204

MarkerSeek score 64.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 2
pi: 0.00204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATGCAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	808-834 bp	1.0	80.3
accD-psaI_p2	TGGATGCAATTGTACCACGT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.8	57.8	798-824 bp	1.0	79.7
accD-psaI_p3	TGGATGCAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.8	58.2	790-816 bp	1.0	79.5
accD-psaI_p4	TGGATGCAATTGTACCACGT	CATTGCAATTGCCGGAAAGA	45.0	45.0	57.8	57.6	801-827 bp	1.0	78.3
accD-psaI_p5	TGGATGCAATTGTACCACGT	ATTGCAATTGCCGGAAAGAC	45.0	45.0	57.8	57.6	800-826 bp	1.0	78.3