Hotspot

atpB-rbcL

LSC · 54092-54894 · 803 bp

Back to Barbarea

Pi 0.00664

MarkerSeek score 63.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 8
pi: 0.00664
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	949-951 bp	1.0	72.2
atpB-rbcL_p2	GGCGATACGTCCCAGATTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	924-926 bp	1.0	72.1
atpB-rbcL_p3	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	891-893 bp	1.0	72.0
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	948-950 bp	1.0	71.9
atpB-rbcL_p5	GGCGATACGTCCCAGATTTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	923-925 bp	1.0	71.9