Hotspot

ndhC-trnV-UAC

LSC · 50210-51116 · 907 bp

Back to Barbarea

Pi 0.00964
MarkerSeek score 59.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
907
variable_sites
13
pi
0.00964
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 980-1000 bp 1.0 68.5
ndhC-trnV-UAC_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 979-999 bp 1.0 66.5
ndhC-trnV-UAC_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 980-1000 bp 1.0 66.4
ndhC-trnV-UAC_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 1008-1028 bp 1.0 65.4
ndhC-trnV-UAC_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 1008-1028 bp 1.0 65.2