Hotspot

psaA-ycf3

LSC · 41786-42456 · 671 bp

Back to Barbarea

Pi 0.00797

MarkerSeek score 64.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 8
pi: 0.00797
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	781-783 bp	1.0	80.0
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	798-800 bp	1.0	79.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGCTTTGAATAA	50.0	45.0	57.9	57.9	769-771 bp	1.0	79.4
psaA-ycf3_p4	AGAAATGACCGGGTTTAGCC	TCACGAGGCGCTTTGAATAA	50.0	45.0	57.9	57.9	786-788 bp	1.0	79.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	789-791 bp	1.0	78.1