Hotspot

trnT-GGU-psbD

LSC · 31446-32686 · 1241 bp

Back to Barbarea

Pi 0.00538

MarkerSeek score 60.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 10
pi: 0.00538
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1393-1396 bp	1.0	80.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1396-1399 bp	1.0	79.8
trnT-GGU-psbD_p3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1415-1418 bp	1.0	79.8
trnT-GGU-psbD_p4	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1410-1413 bp	1.0	79.6
trnT-GGU-psbD_p5	ACCTATCGACTCAGCCAAGA	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.9	58.0	1441-1444 bp	1.0	79.3