Hotspot

trnH-GUG-psbA

LSC · 77-368 · 292 bp

Back to Barbarea

Pi 0.0297
MarkerSeek score 71.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
292
variable_sites
13
pi
0.0297
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 350-352 bp 1.0 81.5
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC GCTGTTGAGGCTCCATCTAC 50.0 55.0 58.2 58.1 351-353 bp 1.0 81.2
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT GTAACGCTCACAACTTCCCA 50.0 50.0 57.9 58.1 382-384 bp 1.0 80.6
trnH-GUG-psbA_p4 CTGCCTTAATCCACTTGGCT TAACGCTCACAACTTCCCAC 50.0 50.0 57.9 58.1 381-383 bp 1.0 80.6
trnH-GUG-psbA_p5 ACTGCCTTAATCCACTTGGC TAACGCTCACAACTTCCCAC 50.0 50.0 58.2 58.1 382-384 bp 1.0 80.4