Hotspot

rpoC1

LSC · 17231-20063 · 2833 bp

Back to Baptisia

Pi 0.000706
MarkerSeek score 56.40
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2833
variable_sites
2
pi
0.000706
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 ACGATCTTTGGCTCTGGAAC AAGACCTGAATTCACACCGG 50.0 50.0 57.9 57.8 2980-2981 bp 1.0 77.8
rpoC1_p2 ACGATCTTTGGCTCTGGAAC CAACCGGGAAGACCTGAATT 50.0 50.0 57.9 57.8 2988-2989 bp 1.0 77.7
rpoC1_p3 ACGATCTTTGGCTCTGGAAC CCGGGAAGACCTGAATTCAC 50.0 55.0 57.9 58.3 2985-2986 bp 1.0 77.2
rpoC1_p4 ACGATCTTTGGCTCTGGAAC CGGGAAGACCTGAATTCACA 50.0 50.0 57.9 57.5 2984-2985 bp 1.0 75.5
rpoC1_p5 CGATCTTTGGCTCTGGAACT AAGACCTGAATTCACACCGG 50.0 50.0 57.6 57.8 2979-2980 bp 1.0 75.5