Hotspot

rpl16

LSC · 79371-80891 · 1521 bp

Back to Baptisia

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCATCCTAAAGACCTCTTTCC	AGGTTCCATATTTCGATAAAACA	45.5	30.4	57.8	54.1	1651-1656 bp	1.0	40.1
rpl16_p2	TGCATCCTAAAGACCTCTTTCC	TCTTTAAGGTTCCATATTTCGA	45.5	31.8	57.8	52.9	1657-1662 bp	1.0	40.1
rpl16_p3	TGCATCCTAAAGACCTCTTTCC	AAGGTTCCATATTTCGATAAAACA	45.5	29.2	57.8	54.8	1652-1657 bp	1.0	40.1
rpl16_p4	TGCATCCTAAAGACCTCTTTCC	AGGTTCCATATTTCGATAAAACAA	45.5	29.2	57.8	54.8	1651-1656 bp	1.0	40.1
rpl16_p5	GCATCCTAAAGACCTCTTTCCT	AGGTTCCATATTTCGATAAAACA	45.5	30.4	57.5	54.1	1650-1655 bp	1.0	40.1