Hotspot

petD

LSC · 74481-75679 · 1199 bp

Back to Baptisia

Pi 0.00167

MarkerSeek score 59.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1199
variable_sites: 2
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GCTTGGGGGAGGAACAATAG	CGTCTCAAAGTGAATTCTCCCT	55.0	45.5	57.9	58.1	1383-1384 bp	1.0	63.2
petD_p2	GCTTGGGGGAGGAACAATAG	TCGTCTCAAAGTGAATTCTCCC	55.0	45.5	57.9	58.1	1384-1385 bp	1.0	63.1
petD_p3	GCTTGGGGGAGGAACAATAG	CGTCTCAAAGTGAATTCTCCC	55.0	47.6	57.9	56.6	1383-1384 bp	1.0	60.8
petD_p4	ATTTATCGCTTGGGGGAGGA	CGTCTCAAAGTGAATTCTCCCT	50.0	45.5	58.5	58.1	1390-1391 bp	1.0	59.8
petD_p5	ATTTATCGCTTGGGGGAGGA	TCGTCTCAAAGTGAATTCTCCC	50.0	45.5	58.5	58.1	1391-1392 bp	1.0	59.7