Hotspot

petB

LSC · 72925-74332 · 1408 bp

Back to Baptisia

Pi 0.00071

MarkerSeek score 59.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1408
variable_sites: 1
pi: 0.00071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTTTGGTAGTTCGACCGTGG	CTATTGTTCCTCCCCCAAGC	50.0	55.0	58.1	57.9	1515 bp	1.0	78.5
petB_p2	GGTAGTTCGACCGTGGAATT	CTATTGTTCCTCCCCCAAGC	50.0	55.0	57.6	57.9	1511 bp	1.0	76.6
petB_p3	TTTTGGTAGTTCGACCGTGG	TCCTCCCCCAAGCGATAAAT	50.0	50.0	58.1	58.5	1508 bp	1.0	75.1
petB_p4	TGGTAGTTCGACCGTGGAAT	CTATTGTTCCTCCCCCAAGC	50.0	55.0	58.7	57.9	1512 bp	1.0	73.7
petB_p5	TTTTGGTAGTTCGACCGTGG	TTCCTCCCCCAAGCGATAAA	50.0	50.0	58.1	58.7	1509 bp	1.0	73.3