Hotspot

petA-psbJ

LSC · 60598-61784 · 1187 bp

Back to Baptisia

Pi 0.00676

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1187
variable_sites: 8
pi: 0.00676
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ACTGGCATTACTGTGATCGG	50.0	50.0	57.8	57.7	1372-1373 bp	1.0	76.2
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	CTGGCATTACTGTGATCGGT	50.0	50.0	57.8	57.7	1371-1372 bp	1.0	76.2
petA-psbJ_p3	CGTTACGTGTCCAAGGTCTC	ACTGGCATTACTGTGATCGG	55.0	50.0	58.3	57.7	1377-1378 bp	1.0	75.7
petA-psbJ_p4	CGTTACGTGTCCAAGGTCTC	CTGGCATTACTGTGATCGGT	55.0	50.0	58.3	57.7	1376-1377 bp	1.0	75.7
petA-psbJ_p5	GATCCGTTACGTGTCCAAGG	CTGGCATTACTGTGATCGGT	55.0	50.0	58.4	57.7	1380-1381 bp	1.0	75.2