Hotspot

trnQ-UUG-accD

LSC · 54003-55836 · 1834 bp

Back to Baptisia

Pi 0.00164
MarkerSeek score 59.00
Primer available yes
Label trnQ-UUG-accD

Feature Metadata

label_name
trnQ-UUG-accD
feature_type
igs
region
LSC
length_bp
1834
variable_sites
3
pi
0.00164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-accD_p1 GAGGTTCGAATCCTTCCGTC CCACACCTGTATTCTAACTCCC 55.0 50.0 58.1 57.9 1917 bp 1.0 62.5
trnQ-UUG-accD_p2 GAGGTTCGAATCCTTCCGTC CCCACACCTGTATTCTAACTCC 55.0 50.0 58.1 57.9 1918 bp 1.0 62.5
trnQ-UUG-accD_p3 GAGGTTCGAATCCTTCCGTC ACACCATAGAATTGAACCACCA 55.0 40.9 58.1 57.6 1889 bp 1.0 60.5
trnQ-UUG-accD_p4 TTCGGAGGTTCGAATCCTTC CCACACCTGTATTCTAACTCCC 50.0 50.0 57.3 57.9 1921 bp 1.0 57.7
trnQ-UUG-accD_p5 TTCGGAGGTTCGAATCCTTC CCCACACCTGTATTCTAACTCC 50.0 50.0 57.3 57.9 1922 bp 1.0 57.7