Hotspot

psbK-trnQ-UUG

LSC · 53378-53930 · 553 bp

Back to Baptisia

Pi 0.00181
MarkerSeek score 59.00
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
553
variable_sites
1
pi
0.00181
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 TAGGCTTCGGGTAATTTCGC GACCAAAACCCGTTGCCTTA 50.0 50.0 58.1 58.7 679-680 bp 1.0 74.4
psbK-trnQ-UUG_p2 TAGGCTTCGGGTAATTTCGC GAATAGCGGGACCAAAACCC 50.0 55.0 58.1 58.9 688-689 bp 1.0 72.6
psbK-trnQ-UUG_p3 GCGTAGGCTTCGGGTAATTT GACCAAAACCCGTTGCCTTA 50.0 50.0 58.3 58.7 682-683 bp 1.0 72.1
psbK-trnQ-UUG_p4 TAGGCTTCGGGTAATTTCGC ACCAAAACCCGTTGCCTTAC 50.0 50.0 58.1 59.0 678-679 bp 1.0 72.1
psbK-trnQ-UUG_p5 TCGGGTAATTTCGCCAAGAA GACCAAAACCCGTTGCCTTA 45.0 50.0 57.5 58.7 673-674 bp 1.0 71.0