Hotspot

petN-psbM

LSC · 36046-37371 · 1326 bp

Back to Baptisia

Pi 0.00077

MarkerSeek score 61.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1326
variable_sites: 1
pi: 0.00077
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1453-1481 bp	1.0	70.3
petN-psbM_p2	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1409-1437 bp	1.0	67.4
petN-psbM_p3	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1410-1438 bp	1.0	67.4
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1453-1481 bp	1.0	66.1
petN-psbM_p5	ATAAGTCTTGCTTGGGCTGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.5	57.6	1458-1486 bp	1.0	66.1