Hotspot

atpI-atpH

LSC · 26294-26980 · 687 bp

Back to Baptisia

Pi 0.00291

MarkerSeek score 60.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 2
pi: 0.00291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	849-851 bp	1.0	74.0
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.3	58.0	761-763 bp	1.0	74.0
atpI-atpH_p3	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	885-887 bp	1.0	73.8
atpI-atpH_p4	ACAAGCGGTATTCAAGCTCT	ATAACGGAAGCGGCAGAAAT	45.0	45.0	57.2	58.0	797-799 bp	1.0	73.7
atpI-atpH_p5	GCTGCGGCTTATATAGGTGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.3	58.2	762-764 bp	1.0	72.8