Hotspot

trnC-GCA-rpoB

LSC · 13300-13984 · 685 bp

Back to Baptisia

Pi 0.00146

MarkerSeek score 46.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTCTTGTTGATCAGGCGACA	ATTTCCTCATTTCCACCCCC	50.0	50.0	57.9	57.4	794 bp	1.0	75.0
trnC-GCA-rpoB_p2	TCTTGTTGATCAGGCGACAC	ATTTCCTCATTTCCACCCCC	50.0	50.0	58.2	57.4	793 bp	1.0	74.1
trnC-GCA-rpoB_p3	AAAAGGATTTGCAGTCCCCC	ATTTCCTCATTTCCACCCCC	50.0	50.0	58.4	57.4	755 bp	1.0	72.9
trnC-GCA-rpoB_p4	TCTCTTGTTGATCAGGCGAC	ATTTCCTCATTTCCACCCCC	50.0	50.0	57.6	57.4	795 bp	1.0	72.7
trnC-GCA-rpoB_p5	CTCTTGTTGATCAGGCGACA	TTCCTCATTTCCACCCCCAA	50.0	50.0	57.9	58.8	792 bp	1.0	72.7