Hotspot

psaC

SSC · 126422-126667 · 246 bp

Back to Baphia

Pi 0.516
MarkerSeek score 76.00
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
114
pi
0.516
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 ATCTATTGCAGCCGCTGAAG CCGCGCTCAAAATGGAAAAT 50.0 45.0 58.4 58.0 688 bp 0.5 56.7
psaC_p2 ATCTATTGCAGCCGCTGAAG AAAACAGAACTTCCCGCAGT 50.0 45.0 58.4 57.9 1251 bp 0.5 56.3
psaC_p3 TGGTCAATGATGCTCGAACA CCGCGCTCAAAATGGAAAAT 45.0 45.0 57.5 58.0 894 bp 0.5 56.2
psaC_p4 TGGTCAATGATGCTCGAACA AAAACAGAACTTCCCGCAGT 45.0 45.0 57.5 57.9 1457 bp 0.5 55.8
psaC_p5 ATCTATTGCAGCCGCTGAAG GAACTTCCCGCAGTGTACAA 50.0 50.0 58.4 58.1 1245 bp 0.5 55.7