Hotspot

ndhF

SSC · 131411-133648 · 2238 bp

Back to Baphia

Pi 0.478
MarkerSeek score 80.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2238
variable_sites
970
pi
0.478
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGGCGAATAAGAAAGAACCTCA AGGGCCCTCTTATCTCTTCC 40.9 55.0 57.7 57.9 2668 bp 0.5 40.9
ndhF_p2 TGGCGAATAAGAAAGAACCTCA GGGCCCTCTTATCTCTTCCT 40.9 55.0 57.7 57.9 2667 bp 0.5 40.9
ndhF_p3 ACTGGCGAATAAGAAAGAACCT AGGGCCCTCTTATCTCTTCC 40.9 55.0 57.7 57.9 2670 bp 0.5 40.8
ndhF_p4 ACTGGCGAATAAGAAAGAACCT GGGCCCTCTTATCTCTTCCT 40.9 55.0 57.7 57.9 2669 bp 0.5 40.8
ndhF_p5 TGGCGAATAAGAAAGAACCTCA GTCGTTGTGGTCGGACTTTA 40.9 50.0 57.7 57.9 2715 bp 0.5 40.6