Hotspot

ndhI-ndhG

SSC · 124665-125114 · 450 bp

Back to Baphia

Pi 0.474
MarkerSeek score 79.10
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
450
variable_sites
191
pi
0.474
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 AATCAAATTGCTTTGGGCCG CAAGACCCGATCCCAGAAAG 45.0 55.0 57.9 58.0 583 bp 0.5 59.0
ndhI-ndhG_p2 AATCAAATTGCTTTGGGCCG CAAGAACCAGTCCCAACGAA 45.0 50.0 57.9 58.0 655 bp 0.5 58.9
ndhI-ndhG_p3 AATCAAATTGCTTTGGGCCG AAGACCCGATCCCAGAAAGA 45.0 50.0 57.9 58.0 582 bp 0.5 58.8
ndhI-ndhG_p4 AATCAAATTGCTTTGGGCCG AACGGTTCAGGCAAATCCAT 45.0 45.0 57.9 58.1 545 bp 0.5 58.6
ndhI-ndhG_p5 TATGGTCAACAAACCGTCCG CAAGACCCGATCCCAGAAAG 50.0 55.0 58.2 58.0 964 bp 0.5 58.1