Hotspot

rps3-rps19

LSC · 86348-86866 · 519 bp

Back to Baphia

Pi 0.124
MarkerSeek score 81.00
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
519
variable_sites
64
pi
0.124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CCAAGTCTGAAACCAAGTGGA TAAACTTCCGAGGACATGCG 47.6 50.0 58.1 58.0 604-656 bp 1.0 79.8
rps3-rps19_p2 CCAAGTCTGAAACCAAGTGGA TCCGAGGACATGCGAAAAAT 47.6 45.0 58.1 57.9 598-650 bp 1.0 79.0
rps3-rps19_p3 CCAAGTCTGAAACCAAGTGGA TTCCGAGGACATGCGAAAAA 47.6 45.0 58.1 58.1 599-651 bp 1.0 78.9
rps3-rps19_p4 CCAAGTCTGAAACCAAGTGGA CCGAGGACATGCGAAAAATG 47.6 50.0 58.1 57.8 597-649 bp 1.0 78.4
rps3-rps19_p5 ACCAAGTCTGAAACCAAGTGG TAAACTTCCGAGGACATGCG 47.6 50.0 58.3 58.0 605-657 bp 1.0 77.6