Hotspot

psbE-petL

LSC · 66165-67365 · 1201 bp

Back to Baphia

Pi 0.124
MarkerSeek score 83.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1201
variable_sites
142
pi
0.124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGATATCCGTCCGAATGGGA AGAACACGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 2386-2414 bp 1.0 88.9
psbE-petL_p2 TGGTCATTGGAGCCTCCTAA AGAACACGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 2044-2098 bp 1.0 88.7
psbE-petL_p3 TGATATCCGTCCGAATGGGA AGAGCGTGATTCCATTCGTC 50.0 50.0 58.0 58.1 2341-2369 bp 1.0 88.7
psbE-petL_p4 TGATATCCGTCCGAATGGGA GAGCGTGATTCCATTCGTCT 50.0 50.0 58.0 58.1 2340-2368 bp 1.0 88.7
psbE-petL_p5 ATGGAATTCCTTGTCGGCTC AGAACACGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 1969 bp 0.5 59.4