Hotspot

petA-psbJ

LSC · 64231-65359 · 1129 bp

Back to Baphia

Pi 0.0683
MarkerSeek score 80.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1129
variable_sites
76
pi
0.0683
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGGCACAAATCTTTTTGGTTCT CATATTCCGGATTGGGCTCA 36.4 50.0 57.2 57.4 1229-1303 bp 1.0 59.3
petA-psbJ_p2 TGGCACAAATCTTTTTGGTTCT TATTCCGGATTGGGCTCATC 36.4 50.0 57.2 57.1 1227-1301 bp 1.0 56.9
petA-psbJ_p3 TGGCACAAATCTTTTTGGTTCT TCATATTCCGGATTGGGCTC 36.4 50.0 57.2 57.1 1230-1304 bp 1.0 56.9
petA-psbJ_p4 TGGCACAAATCTTTTTGGTTCTT CATATTCCGGATTGGGCTCA 34.8 50.0 57.9 57.4 1229-1303 bp 1.0 56.5
petA-psbJ_p5 TGGCACAAATCTTTTTGGTTC CATATTCCGGATTGGGCTCA 38.1 50.0 55.8 57.4 1229-1303 bp 1.0 55.5