Hotspot

trnG-UCC-trnS-GCU

LSC · 55206-55770 · 565 bp

Back to Baphia

Pi 0.0788
MarkerSeek score 82.50
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
565
variable_sites
42
pi
0.0788
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCACACTTTTACCACT CAATCCGACGCTTTAGTCCA 38.1 50.0 55.5 58.0 634-826 bp 1.0 59.8
trnG-UCC-trnS-GCU_p2 ACGAATCACACTTTTACCACT AATCCGACGCTTTAGTCCAC 38.1 50.0 55.5 58.0 633-825 bp 1.0 59.8
trnG-UCC-trnS-GCU_p3 AGAACGAATCACACTTTTACCAC CAATCCGACGCTTTAGTCCA 39.1 50.0 57.3 58.0 637-829 bp 1.0 58.7
trnG-UCC-trnS-GCU_p4 GAACGAATCACACTTTTACCACT CAATCCGACGCTTTAGTCCA 39.1 50.0 57.3 58.0 636-828 bp 1.0 58.7
trnG-UCC-trnS-GCU_p5 GAACGAATCACACTTTTACCACT AATCCGACGCTTTAGTCCAC 39.1 50.0 57.3 58.0 635-827 bp 1.0 58.7