Pi
0.0679
MarkerSeek score
83.70
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 1082-1217 bp | 1.0 | 86.7 |
| trnL-UAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 1090-1225 bp | 1.0 | 86.1 |
| trnL-UAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 1114-1249 bp | 1.0 | 85.6 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 1122-1257 bp | 1.0 | 85.1 |
| trnL-UAA-trnT-UGU_p5 | AGTCCGTAGCGTCTACCAAT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.2 | 57.9 | 1096-1231 bp | 1.0 | 84.7 |