Hotspot

ndhJ-trnF-GAA

LSC · 12572-13377 · 806 bp

Back to Baphia

Pi 0.0851
MarkerSeek score 86.00
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
806
variable_sites
64
pi
0.0851
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 TTGGATAGGATGGCCTTTGC GGGATAGCTCAGCTGGTAGA 50.0 55.0 57.9 58.0 929-946 bp 1.0 88.9
ndhJ-trnF-GAA_p2 TTGGATAGGATGGCCTTTGC CCTCGTGTCACCAGTTCAAA 50.0 50.0 57.9 58.1 893-910 bp 1.0 88.7
ndhJ-trnF-GAA_p3 TTGGATAGGATGGCCTTTGC CTGGTAGAGCAGAGGACTGA 50.0 55.0 57.9 57.9 917-934 bp 1.0 87.9
ndhJ-trnF-GAA_p4 ATGCCCGAAAGTTGGATAGG GGGATAGCTCAGCTGGTAGA 50.0 55.0 57.7 58.0 940-957 bp 1.0 86.6
ndhJ-trnF-GAA_p5 ATGCCCGAAAGTTGGATAGG CCTCGTGTCACCAGTTCAAA 50.0 50.0 57.7 58.1 904-921 bp 1.0 86.4