Hotspot

ycf37

Genome · 49365-49901 · 537 bp

Pi 0.306

MarkerSeek score 79.50

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 537
variable_sites: 161
pi: 0.306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	TTTGAGCCTTCCAGTGTTCC	ACGGGATATAGCGCAGTTTG	50.0	50.0	58.0	58.1	812 bp	0.5	58.8
ycf37_p2	TTTGAGCCTTCCAGTGTTCC	ACAGTAGGCGAGAGTTCTGA	50.0	50.0	58.0	57.8	1361 bp	0.5	58.2
ycf37_p3	GCGTCCACTAGGTGTTTTCA	ACGGGATATAGCGCAGTTTG	50.0	50.0	58.1	58.1	870 bp	0.5	57.9
ycf37_p4	TTTGAGCCTTCCAGTGTTCC	GGGATATAGCGCAGTTTGGT	50.0	50.0	58.0	57.7	810 bp	0.5	57.8
ycf37_p5	AGCGTCCACTAGGTGTTTTC	ACGGGATATAGCGCAGTTTG	50.0	50.0	57.8	58.1	871 bp	0.5	57.6