Hotspot

syfB

Genome · 181491-183587 · 2097 bp

Pi 0.286

MarkerSeek score 78.10

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2097
variable_sites: 599
pi: 0.286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	AACTGGTAAGGGTGCAAAGG	CGAGATGGTAGCACAGGAAC	50.0	55.0	58.0	58.1	2760 bp	0.5	59.4
syfB_p2	GTGCCACAGAAATAAACCGC	CGAGATGGTAGCACAGGAAC	50.0	55.0	58.0	58.1	2796 bp	0.5	59.2
syfB_p3	AACTGGTAAGGGTGCAAAGG	AAGGTCTCGAGATGGTAGCA	50.0	50.0	58.0	57.9	2767 bp	0.5	58.8
syfB_p4	AACTGGTAAGGGTGCAAAGG	TCGAGATGGTAGCACAGGAA	50.0	50.0	58.0	58.2	2761 bp	0.5	58.7
syfB_p5	GTGCCACAGAAATAAACCGC	AAGGTCTCGAGATGGTAGCA	50.0	50.0	58.0	57.9	2803 bp	0.5	58.7