Hotspot

rps2

Genome · 93710-94435 · 726 bp

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Pi 0.166
MarkerSeek score 79.30
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
Genome
length_bp
726
variable_sites
120
pi
0.166
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 CTGGTCGACGAGCTACAAAA ACGGAAGCTGCTATTTCTGG 50.0 50.0 57.9 58.0 1445 bp 0.5 59.3
rps2_p2 CTGGTCGACGAGCTACAAAA CGGAAGCTGCTATTTCTGGT 50.0 50.0 57.9 58.0 1444 bp 0.5 59.3
rps2_p3 CTGGTCGACGAGCTACAAAA GCCGCAAGTTTTCAAGAGAC 50.0 50.0 57.9 58.0 1478 bp 0.5 59.2
rps2_p4 GCTTTTACAGTCTGAGCGGA ACGGAAGCTGCTATTTCTGG 50.0 50.0 57.9 58.0 1209 bp 0.5 59.1
rps2_p5 GCTTTTACAGTCTGAGCGGA CGGAAGCTGCTATTTCTGGT 50.0 50.0 57.9 58.0 1208 bp 0.5 59.1