Hotspot

rne

Genome · 63142-64677 · 1536 bp

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Pi 0.209
MarkerSeek score 78.70
Primer available yes
Label rne

Feature Metadata

label_name
rne
feature_type
gene
region
Genome
length_bp
1536
variable_sites
321
pi
0.209
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rne_p1 GGAGCTACAGTACAGGCAAC TTCCAAGCTGAGCACAACAT 55.0 45.0 58.0 58.0 2780 bp 0.5 59.8
rne_p2 GGAGCTACAGTACAGGCAAC GCCTGTAGTTGGTATCTGGC 55.0 55.0 58.0 58.0 2502 bp 0.5 59.5
rne_p3 GGAGCTACAGTACAGGCAAC TCCGGTGAATCCTTACCAGT 55.0 50.0 58.0 58.1 2303 bp 0.5 59.5
rne_p4 GGAGCTACAGTACAGGCAAC CCCAATTGGCCAAGGTAGTT 55.0 50.0 58.0 58.1 2853 bp 0.5 59.4
rne_p5 GGAGCTACAGTACAGGCAAC CGTGAAACAAGCGAAAACGA 55.0 45.0 58.0 57.9 2663 bp 0.5 59.3