Hotspot

rbcR

Genome · 59803-60756 · 954 bp

Pi 0.143

MarkerSeek score 79.10

Primer available yes

Label rbcR

Feature Metadata

label_name: rbcR
feature_type: gene
region: Genome
length_bp: 954
variable_sites: 136
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR_p1	TTTGACTTCTGCCCGAAACA	CCAGTTAGTACGGACTTGGC	45.0	55.0	58.0	58.0	2311 bp	0.5	59.6
rbcR_p2	TTTGACTTCTGCCCGAAACA	ATGCAGCTGCTAAAACTGGT	45.0	45.0	58.0	58.1	1938 bp	0.5	59.0
rbcR_p3	CTTCTGCCCGAAACACATCT	CCAGTTAGTACGGACTTGGC	50.0	55.0	58.2	58.0	2306 bp	0.5	58.5
rbcR_p4	CTTCTGCCCGAAACACATCT	ATGCAGCTGCTAAAACTGGT	50.0	45.0	58.2	58.1	1933 bp	0.5	57.8
rbcR_p5	CCCTGAGAGCTAGGCCAATA	CCAGTTAGTACGGACTTGGC	55.0	55.0	58.3	58.0	2190 bp	0.5	57.7